Journal:

Article Title: Evaluation of Three Techniques for Detection of Low-Level Methicillin-Resistant Staphylococcus aureus (MRSA): a Disk Diffusion Method with Cefoxitin and Moxalactam, the Vitek 2 System, and the MRSA-Screen Latex Agglutination Test

doi: 10.1128/JCM.40.8.2766-2771.2002

Figure Lengend Snippet: Gel image of representative PCR mec gene products, mecA (528 bp), mecRA (310 bp), mecRB (236 bp), and mecI (481 bp), from different MRSA isolates, obtained with specific primers. Lane M, DNA molecular weight markers. Lanes 1, 7, 12, and 17, MRSA strain ATCC 44300; lanes 2, 8, and 13, MRSA strain CCUG 31966; lane 3, BORSA strain CCUG 35302; lanes 4, 9, 14, and 18, MRSA isolate 88; lanes 5, 10, and 15, MRSA isolate 112; lanes 6, 11, and 16, MRSA isolate 105.

Article Snippet: Control strains used for all assays included the MSSA β-lactamase-negative strain ATCC 25923, the BORSA (β-lactamase-positive, borderline methicillin-resistant, mecA -negative S. aureus ) strain CCUG 35302, the heterogeneous class 1 MRSA strain ATCC 43300 T , and the homogeneous MRSA strain CCUG 31966 from the Swedish Collection.

Techniques: Molecular Weight

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Clinical carbapenemase-producing and -nonproducing Enterobacteriaceae strains analysed in this study.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: A) The spectrum obtained for the pure meropenem (arrived with ice) showed the peaks corresponding to the intact drug (384 ±1 Da) and to its sodium salts (406 ±1 Da; 428 ±1 Da); the spectrum obtained for the carbapenemase-nonproducing reference strain ( K . pneumoniae ATCC BAA-1706) showed the same peaks as the pure meropenem, while the 2 carbapenemase-producing reference strains ( K . pneumoniae ATCC BAA-1705, bla KPC positive, and K . pneumoniae ATCC BAA-2146, bla NDM positive) gave the peaks corresponding to the meropenem degradation (358 ±1 Da, the decarboxylated product) and to its sodium salts (380 ±1 Da; 402 ±1 Da; 424 ±1 Da). B) Example of spectra obtained for clinical samples tested with pure meropenem arrived with ice: a carbapenemase-nonproducing strain and two carbapenemase-producing strains, one of which ( K . pneumoniae No. 1135 strain) showing the peaks corresponding to both the pure meropenem and the degradation products of the drug.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques:

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Results of Meropenem Hydrolysis Assay (MHA) in case of intact or degraded drug in 1127 Enterobacteriaceae strains included in the panels I and II.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques: Hydrolysis Assay

Journal: PLoS ONE

Article Title: Evaluation of a modified meropenem hydrolysis assay on a large cohort of KPC and VIM carbapenemase-producing Enterobacteriaceae

doi: 10.1371/journal.pone.0174908

Figure Lengend Snippet: Results of Meropenem Hydrolysis Assay (MHA) in case of intact or degraded drug in 58 Enterobacteriaceae strains included in the panel III.

Article Snippet: This study included 8 reference strains received from American Type Culture Collection (ATCC), Public Health England’s National Collection of Type Cultures (NCTC) or United Kingdom External Quality Assessment Service (UK NEQAS): 3 K . pneumoniae carbapenemase-producing strains (ATCC BAA-1705, bla KPC positive, ATCC BAA-2146, bla NDM positive, and NCTC 13442, bla OXA-48 positive), 1 K . pneumoniae carbapenemase-nonproducing strain (ATCC BAA-1706), 2 AmpC strains ( Escherichia coli NEQAS 2832 and Enterobacter cloacae NEQAS 3419) and 2 ESBL strains ( E . coli NEQAS 3253 and Proteus mirabilis NEQAS 3341).

Techniques: Hydrolysis Assay

Journal: PLoS ONE

Article Title: Faecal carriage of CTX-M extended-spectrum beta-lactamase-producing Enterobacteriaceae among street children dwelling in Mwanza city, Tanzania

doi: 10.1371/journal.pone.0184592

Figure Lengend Snippet: Proportion of antimicrobial resistance and beta-lactamase genes among 36 ESBL producing E . coli and K . pneumoniae .

Article Snippet: E . coli ATCC 25922 (non-beta-lactamase-producing strain) and E . coli ATCC 35218 (beta-lactamase-producing strain) were used for quality control.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Primer Sequences Used for Detection Extended-Spectrum Beta-Lactamase and Carbapenemase Genes

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Sequencing, Amplification

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Magnitude and Distribution of Carbapenemase Producing Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Comparison of Antimicrobial Resistance Patterns Between ESBLand Carbapenemase Producing and Non-Producing Enterobacteriaceae Isolates at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques: Comparison

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Gel image of the ESBL and carbapenemase genes from Enterobacteriaceae isolates at TASH 2019. ( A ) bla CTX-M (754bp), bla SHV (403bp) and bla TEM (293bp).

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal: Infection and Drug Resistance

Article Title: Prevalence and Molecular Characterization of Extended Spectrum β-Lactamase and Carbapenemase-Producing Enterobacteriaceae Isolates from Bloodstream Infection Suspected Patients in Addis Ababa, Ethiopia

doi: 10.2147/IDR.S349566

Figure Lengend Snippet: Distribution of ESBL and Carbapenemase Genes Among Enterobacteriaceae Isolates from Patients with BSI at TASH, 2019

Article Snippet: For carbapenemase confirmatory tests control strains Klebsiella pneumoniae ATCC ® BAA-1705 (positive) and Escherichia coli ATCC ® 25922 (negative) were used.

Techniques:

Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: Bacterial strains used in this study

Article Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Strain β-Lactamase Inoculum (CFU/ml) MIC (μg/ml) of antimicrobial agent(s) a : CTX CTX-CA CAZ CAZ-CA ATM ATM-CA CPD CPD-CA FEP FEP-CA ATCC 13883 None 10 5 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 10 6 ≤0.12 ≤0.12 0.25 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ATCC 700603 SHV-18 10 5 1 0.5 8 0.5 2 0.25 8 1 0.5 0.5 10 6 4 0.5 8 1 4 0.25 8 2 0.5 0.5 OC4244 SHV-5, TEM-1 10 5 >128 0.25 >128 0.5 >128 ≤0.12 >128 0.5 32 ≤0.12 10 6 >128 0.25 >128 1 >128 ≤0.12 >128 0.5 >128 ≤0.12 OC4239 TEM-6, SHV-1 10 5 0.25 ≤0.12 64 1 32 ≤0.12 16 0.5 2 ≤0.12 10 6 0.5 ≤0.12 >128 2 64 ≤0.12 64 0.25 2 ≤0.12 OC4110 TEM-10 10 5 1 ≤0.12 128 1 64 ≤0.12 32 0.25 2 ≤0.12 10 6 4 ≤0.12 >128 1 128 ≤0.12 >128 0.5 2 ≤0.12 OC4105 TEM-26, SHV-1 10 5 2 ≤0.12 >128 4 128 0.25 16 0.25 8 ≤0.12 10 6 2 ≤0.12 >128 4 128 0.25 32 0.5 16 0.25 OC4074 MIR-1, TEM-1 10 5 8 16 32 32 16 16 64 128 0.5 0.5 10 6 8 16 32 32 16 32 128 >128 1 1 OC4076 b K-1 10 5 1 ≤0.12 0.25 ≤0.12 16 ≤0.12 8 ≤0.12 1 ≤0.12 10 6 2 ≤0.12 0.25 ≤0.12 128 0.25 32 0.25 4 0.25 OC4250 ACT-1, TEM, SHV 10 5 16 32 64 32 32 32 64 64 1 0.5 10 6 32 32 128 64 32 32 >128 >128 2 0.5 OC5064 FOX-5, TEM, SHV 10 5 16 16 64 64 4 4 128 128 1 0.5 10 6 16 32 64 128 8 8 >128 128 1 1 Open in a separate window a The antimicrobial agents were each tested alone and with clavulanic acid (CA) at 4 μg/ml.

Techniques: Functional Assay

Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam

Article Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Strain β-Lactamase Inoculum (CFU/ml) MIC (μg/ml) of antimicrobial agent(s) a : CTX CTX-CA CAZ CAZ-CA ATM ATM-CA CPD CPD-CA FEP FEP-CA ATCC 13883 None 10 5 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 10 6 ≤0.12 ≤0.12 0.25 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ATCC 700603 SHV-18 10 5 1 0.5 8 0.5 2 0.25 8 1 0.5 0.5 10 6 4 0.5 8 1 4 0.25 8 2 0.5 0.5 OC4244 SHV-5, TEM-1 10 5 >128 0.25 >128 0.5 >128 ≤0.12 >128 0.5 32 ≤0.12 10 6 >128 0.25 >128 1 >128 ≤0.12 >128 0.5 >128 ≤0.12 OC4239 TEM-6, SHV-1 10 5 0.25 ≤0.12 64 1 32 ≤0.12 16 0.5 2 ≤0.12 10 6 0.5 ≤0.12 >128 2 64 ≤0.12 64 0.25 2 ≤0.12 OC4110 TEM-10 10 5 1 ≤0.12 128 1 64 ≤0.12 32 0.25 2 ≤0.12 10 6 4 ≤0.12 >128 1 128 ≤0.12 >128 0.5 2 ≤0.12 OC4105 TEM-26, SHV-1 10 5 2 ≤0.12 >128 4 128 0.25 16 0.25 8 ≤0.12 10 6 2 ≤0.12 >128 4 128 0.25 32 0.5 16 0.25 OC4074 MIR-1, TEM-1 10 5 8 16 32 32 16 16 64 128 0.5 0.5 10 6 8 16 32 32 16 32 128 >128 1 1 OC4076 b K-1 10 5 1 ≤0.12 0.25 ≤0.12 16 ≤0.12 8 ≤0.12 1 ≤0.12 10 6 2 ≤0.12 0.25 ≤0.12 128 0.25 32 0.25 4 0.25 OC4250 ACT-1, TEM, SHV 10 5 16 32 64 32 32 32 64 64 1 0.5 10 6 32 32 128 64 32 32 >128 >128 2 0.5 OC5064 FOX-5, TEM, SHV 10 5 16 16 64 64 4 4 128 128 1 0.5 10 6 16 32 64 128 8 8 >128 128 1 1 Open in a separate window a The antimicrobial agents were each tested alone and with clavulanic acid (CA) at 4 μg/ml.

Techniques:

Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: K. pneumoniae MIC values for extended-spectrum cephalosporins and aztreonam

Article Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Strain β-Lactamase Inoculum (CFU/ml) MIC (μg/ml) of antimicrobial agent(s) a : CTX CTX-CA CAZ CAZ-CA ATM ATM-CA CPD CPD-CA FEP FEP-CA ATCC 13883 None 10 5 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 10 6 ≤0.12 ≤0.12 0.25 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ATCC 700603 SHV-18 10 5 1 0.5 8 0.5 2 0.25 8 1 0.5 0.5 10 6 4 0.5 8 1 4 0.25 8 2 0.5 0.5 OC4244 SHV-5, TEM-1 10 5 >128 0.25 >128 0.5 >128 ≤0.12 >128 0.5 32 ≤0.12 10 6 >128 0.25 >128 1 >128 ≤0.12 >128 0.5 >128 ≤0.12 OC4239 TEM-6, SHV-1 10 5 0.25 ≤0.12 64 1 32 ≤0.12 16 0.5 2 ≤0.12 10 6 0.5 ≤0.12 >128 2 64 ≤0.12 64 0.25 2 ≤0.12 OC4110 TEM-10 10 5 1 ≤0.12 128 1 64 ≤0.12 32 0.25 2 ≤0.12 10 6 4 ≤0.12 >128 1 128 ≤0.12 >128 0.5 2 ≤0.12 OC4105 TEM-26, SHV-1 10 5 2 ≤0.12 >128 4 128 0.25 16 0.25 8 ≤0.12 10 6 2 ≤0.12 >128 4 128 0.25 32 0.5 16 0.25 OC4074 MIR-1, TEM-1 10 5 8 16 32 32 16 16 64 128 0.5 0.5 10 6 8 16 32 32 16 32 128 >128 1 1 OC4076 b K-1 10 5 1 ≤0.12 0.25 ≤0.12 16 ≤0.12 8 ≤0.12 1 ≤0.12 10 6 2 ≤0.12 0.25 ≤0.12 128 0.25 32 0.25 4 0.25 OC4250 ACT-1, TEM, SHV 10 5 16 32 64 32 32 32 64 64 1 0.5 10 6 32 32 128 64 32 32 >128 >128 2 0.5 OC5064 FOX-5, TEM, SHV 10 5 16 16 64 64 4 4 128 128 1 0.5 10 6 16 32 64 128 8 8 >128 128 1 1 Open in a separate window a The antimicrobial agents were each tested alone and with clavulanic acid (CA) at 4 μg/ml.

Techniques:

Journal:

Article Title: Effects of Inoculum and ?-Lactamase Activity in AmpC- and Extended-Spectrum ?-Lactamase (ESBL)-Producing Escherichia coli and Klebsiella pneumoniae Clinical Isolates Tested by Using NCCLS ESBL Methodology

doi: 10.1128/JCM.42.1.269-275.2004

Figure Lengend Snippet: β-Lactamase hydrolysis parameters a

Article Snippet: E. coli MIC values for extended-spectrum cephalosporins and aztreonam table ft1 table-wrap mode="anchored" t5 TABLE 3. caption a7 Strain β-Lactamase Inoculum (CFU/ml) MIC (μg/ml) of antimicrobial agent(s) a : CTX CTX-CA CAZ CAZ-CA ATM ATM-CA CPD CPD-CA FEP FEP-CA ATCC 13883 None 10 5 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 10 6 ≤0.12 ≤0.12 0.25 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ≤0.12 ATCC 700603 SHV-18 10 5 1 0.5 8 0.5 2 0.25 8 1 0.5 0.5 10 6 4 0.5 8 1 4 0.25 8 2 0.5 0.5 OC4244 SHV-5, TEM-1 10 5 >128 0.25 >128 0.5 >128 ≤0.12 >128 0.5 32 ≤0.12 10 6 >128 0.25 >128 1 >128 ≤0.12 >128 0.5 >128 ≤0.12 OC4239 TEM-6, SHV-1 10 5 0.25 ≤0.12 64 1 32 ≤0.12 16 0.5 2 ≤0.12 10 6 0.5 ≤0.12 >128 2 64 ≤0.12 64 0.25 2 ≤0.12 OC4110 TEM-10 10 5 1 ≤0.12 128 1 64 ≤0.12 32 0.25 2 ≤0.12 10 6 4 ≤0.12 >128 1 128 ≤0.12 >128 0.5 2 ≤0.12 OC4105 TEM-26, SHV-1 10 5 2 ≤0.12 >128 4 128 0.25 16 0.25 8 ≤0.12 10 6 2 ≤0.12 >128 4 128 0.25 32 0.5 16 0.25 OC4074 MIR-1, TEM-1 10 5 8 16 32 32 16 16 64 128 0.5 0.5 10 6 8 16 32 32 16 32 128 >128 1 1 OC4076 b K-1 10 5 1 ≤0.12 0.25 ≤0.12 16 ≤0.12 8 ≤0.12 1 ≤0.12 10 6 2 ≤0.12 0.25 ≤0.12 128 0.25 32 0.25 4 0.25 OC4250 ACT-1, TEM, SHV 10 5 16 32 64 32 32 32 64 64 1 0.5 10 6 32 32 128 64 32 32 >128 >128 2 0.5 OC5064 FOX-5, TEM, SHV 10 5 16 16 64 64 4 4 128 128 1 0.5 10 6 16 32 64 128 8 8 >128 128 1 1 Open in a separate window a The antimicrobial agents were each tested alone and with clavulanic acid (CA) at 4 μg/ml.

Techniques: